biotinylated crp detection antibody  (R&D Systems)

Journal: Talanta

Article Title: Laser-patterned paper-based flow-through filters and lateral flow immunoassays to enable the detection of C-reactive protein.

doi: 10.1016/j.talanta.2021.123056

Figure Lengend Snippet: Fig. 7. Flow-through filter with pre-polymer concentration of 7.7% tested with 20 μL of a sample that contains anti-mouse antibody labelled with 40 nm Au- nanoparticles, 3 μm latex beads labelled with streptavidin and mouse biotinylated IgG antibody (analyte) at concentrations between 0 μg/ml and 500 μg/ml.

Article Snippet: For this we used (as the analyte) human CRP (Sigma-Aldrich with stock concentration of 1 mg/ml, product number C1617), mouse anti-human CRP (acquired from R&D systems with stock concentration of 500 μg/ml, product number MAB17071) labelled with 40 nm Au-nanoparticles (as the detection antibody) and mouse antihuman CRP biotinylated IgG antibody (R&D systems with stock concentration of 50 μg/ml, product number BAM17072) tagged with 3 μm latex beads (white dyed) labelled with streptavidin as the filtration antibody.

Techniques: Polymer, Concentration Assay

Journal: Frontiers in Immunology

Article Title: Preterm Birth Is Associated With Immune Dysregulation Which Persists in Infants Exposed to Histologic Chorioamnionitis

doi: 10.3389/fimmu.2021.722489

Figure Lengend Snippet: Cord blood analytes in preterm infants and term-born controls.

Article Snippet: A third multiplex analysis was developed in-house applying extracts diluted 1:10 in diluent 7 (#R54BB, MSD) using antibodies specific for C3 (HYB030-07 and HYB030-06, SSI Antibodies, Copenhagen, Denmark), C5a (10604-MM04 and 10604-MM06, Sino Biological, Eschborn, Germany), C9 (R-plex kit #F21XZ, MSD), MMP-9 (BAF911 and MAB911), RANTES (MAB278 and AF278NA) and CRP (BAM17072 and MAB1701) (all R&D Systems, Minneapolis, US) for coating the U-plex plate and for detection, respectively.

Techniques:

Journal: Frontiers in Immunology

Article Title: Preterm Birth Is Associated With Immune Dysregulation Which Persists in Infants Exposed to Histologic Chorioamnionitis

doi: 10.3389/fimmu.2021.722489

Figure Lengend Snippet: Day 5 blood analyte concentrations for preterm infants exposed to histologic chorioamnionitis compared to those not exposed.

Article Snippet: A third multiplex analysis was developed in-house applying extracts diluted 1:10 in diluent 7 (#R54BB, MSD) using antibodies specific for C3 (HYB030-07 and HYB030-06, SSI Antibodies, Copenhagen, Denmark), C5a (10604-MM04 and 10604-MM06, Sino Biological, Eschborn, Germany), C9 (R-plex kit #F21XZ, MSD), MMP-9 (BAF911 and MAB911), RANTES (MAB278 and AF278NA) and CRP (BAM17072 and MAB1701) (all R&D Systems, Minneapolis, US) for coating the U-plex plate and for detection, respectively.

Techniques:

Journal: Analytical chemistry

Article Title: Optimized Immobilization of Biomolecules on Nonspherical Gold Nanostructures for Efficient Localized Surface Plasmon Resonance Biosensing.

doi: 10.1021/acs.analchem.9b03780

Figure Lengend Snippet: Figure 5: Functionalization of Au nanostructures with anti-hCRP was followed by incubation in 0.01% BSA (2 hours, RT), 100 ng/mL hCRP (2 hours, 4°C), and biotinylated anti-hCRP (anti- hCRP∗, 1.5 hours, 4°C). UV-Vis spectra were recorded after each protein immobilization step.

Article Snippet: Human CRP antibody (MAB 17073), human CRP biotinylated antibody (BAM 17072), and recombinant human interleukin-6 protein (IL-6, 206-IL/CF) were purchased from R&D Systems (MN, USA).

Techniques: Incubation

Journal: Analytical chemistry

Article Title: Optimized Immobilization of Biomolecules on Nonspherical Gold Nanostructures for Efficient Localized Surface Plasmon Resonance Biosensing.

doi: 10.1021/acs.analchem.9b03780

Figure Lengend Snippet: Figure 6: (a) UV-Vis spectra recorded from bare Au nanostructures (red), after blocking the surface with BSA (blue), after incubation in hCRP (purple), and after incubation in biotinylated anti-hCRP (teal). (b) Control 1: ∆λ LSPR (nm) detected after incubation of BSA-blocked bare substrate in hCRP and biotinylated anti-hCRP. (c) UV-Vis spectra recorded from bare Au nanostructures (red), after blocking the surface with BSA (blue), and after incubation in biotinylated anti-hCRP (teal). (d) Control 2: ∆λ LSPR (nm) after incubation of BSA-blocked bare substrate in biotinylated anti- hCRP.

Article Snippet: Human CRP antibody (MAB 17073), human CRP biotinylated antibody (BAM 17072), and recombinant human interleukin-6 protein (IL-6, 206-IL/CF) were purchased from R&D Systems (MN, USA).

Techniques: Blocking Assay, Incubation, Control

Journal: Analytical chemistry

Article Title: Optimized Immobilization of Biomolecules on Nonspherical Gold Nanostructures for Efficient Localized Surface Plasmon Resonance Biosensing.

doi: 10.1021/acs.analchem.9b03780

Figure Lengend Snippet: Figure 7: Specificity analysis: (a) UV-Vis spectra recorded from bare Au nanostructures (red), after introducing MUA linkers (pink), after incubating in anti-hCRP (green), further blocking with BSA (blue), incubating in non-specific IL-6 (purple), and biotinylated anti-hCRP (teal). (b) ∆λLSPR (nm) averaged over eight runs of incubation of substrates functionalized with anti-hCRP in 100 ng/mL IL-6; anti-hCRP immobilized on Au nanostructures using MUA linkers did not bind to non-specific IL-6 or biotinylated anti-hCRP (anti-hCRP∗).

Article Snippet: Human CRP antibody (MAB 17073), human CRP biotinylated antibody (BAM 17072), and recombinant human interleukin-6 protein (IL-6, 206-IL/CF) were purchased from R&D Systems (MN, USA).

Techniques: Blocking Assay, Incubation